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Objectives To quantify gendered patterns of professional misidentification among hospital doctors.Methods An anonymised cross-sectional survey of hospital doctors was conducted in February 2025 at Basingstoke and North Hampshire Hospital, a UK district general hospital. Respondents reported the frequency, source and type of misidentification, alongside perceived impact on workflow. Responses were analysed by gender using Fisher’s exact test.Results 52 doctors responded (32 female, 20 male). All female respondents reported misidentification at least once (vs 75.0% of men, p=0.006) and were more often misidentified weekly to daily (81.2% vs 25.0%, p<0.001). All women reported being mistaken for nurses (vs 30.0%, p<0.001) and misidentified downward (vs 50.0%, p<0.001); men were more often misidentified upward (65.0% vs 25.0%, p=0.008). Elevating nicknames were more often directed at men (60.0% vs 18.8%, p=0.003). Misidentification disrupted workflow at least sometimes for 40.6% of women versus 5.0% of men (p=0.008).Conclusions In this single-centre cohort, misidentification showed consistent, statistically significant gendered patterns—women downward, men upward—suggesting a broader pattern warranting investigation in larger, multicentre studies. Visible role-labelling, inclusive communication training and leadership modelling merit consideration and future evaluation.