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Introduction Neuropathic pain mainly occurs due to alteration of neuronal micro-tubular proteins and glial cells. Retinoids possess the wound repair processes via fibroblast growth factor (FGF) regulatory actions. However, the role of β-carotene hydrogels (BCHG) in the amelioration of sciatic nerve repairs is not explored in rats.Aim of study The present study is designed to investigate the BCHG effect in sciatic nerve axotomy-induced neuropathic pain behaviour in rats with the connection of FGF-13 function.Method The axotomy of the sciatic nerve (SCT) was made in the sciatic nerve bifurcation site. The BCHG (20 μL of 2.5 and 5%), pregabalin (PG: 20 μL of 1%), and heparin-based hydrogel (HHG: 20 μL of 3%) were loaded in chitosan/keratin (CK) tube. The sciatic nerve functional index (SFI) was analyzed for neuronal functions. The pain-sensitive tests, i.e., thermal and mechanical hyperalgesia were assessed on the different days, i.e., days 0, 1, 7, 14, and 21. The tissue total protein, total calcium, tumor necrosis factor-alpha (TNF-α), and FGF-13 levels were estimated to assess tissue biomarker changes.Results The application of BCHG (2.5 and 5%) improves the neuronal repair process in SCT in rats via regulation of neuronal calcium, TNF-α, and FGF-13 protein expressions. It is also revealed in the neuropathic pain behaviour & neuronal functional test.Conclusion Hence, the BCHG possesses neuronal repair actions in rat SCT and attenuates the neuropathic pain behaviours via FGF-13 activation, anti-TNF-α, and calcium channel regulatory actions.Conflict of Interest No