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Objective There is a growing need for accessible and scientifically robust tools to measure psychosocial risks at work (PRW), particularly considering evolving occupational health legislations and the diverse realities of workplaces, including those of small and medium-sized enterprises (SME). This study aims to evaluate the stability and psychometric properties of the French version of the Questionnaire on Psychosocial Risks, Well-being, and Health at Work (QRBEST) across two complementary samples, supporting its use as a valid tool to assess PRW and their associated outcomes.Methods A cross-sectional study was conducted with 3000 French-speaking workers from a large, demographically diverse populational panel that used quotas to reflect the sociodemographic characteristics of Québec’s working population as well as a second sample of 704 employees from eight Québec SMEs. Both samples completed the QRBEST, assessing 22 PRWs and 12 indicators of well-being, health, and productivity. Data collection took place from October-November 2024 for the panel and August-November 2024 for the SMEs (average participation rate: 72%).Psychometric analyses included internal consistency (Cronbach’s alpha), test-retest reliability (in panel only), item-total correlations, item response theory, and both exploratory and confirmatory factor analyses, conducted separately in both samples.Results In both samples, the QRBEST demonstrated strong psychometric properties. All multi-item scales showed good to excellent internal consistency (α>.75). Exploratory and confirmatory factor analyses supported the expected factor structures and showed excellent model fit (SRMR<0.08; GFI≈1), indicating that the QRBEST performs reliably across different workplace contexts.Conclusion The QRBEST is a scientifically robust instrument for assessing PRW and their associated outcomes. Its strong performance across both general and SME samples supports its practical use in diverse workplaces, making it suitable for a wide range of occupational settings and helping bridge the gap between risk identification and preventive action.Funding Fonds institutionnel de recherche de l’Université du Québec à Rimouski.