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Introduction o introduce a novel recalculation program for Perfusion-Weighted Imaging (PWI) and Diffusion-Weighted Imaging (DWI) aimed at enhancing the accuracy of stroke imaging analysis, particularly in identifying the penumbra and infarct core.Aim of Study The program addresses limitations in current imaging techniques by providing a more tailored and precise assessment of brain perfusion, thereby improving clinical decision-making in acute stroke management.Method The PWI-DWI Recalculation Program employs simple algorithms to recalibrate perfusion maps based on patient-specific data. The program integrates directly with raw imaging data inputs (PWI and DWI) and applies preprocessing, recalibration, and mismatch calculation steps to generate improved perfusion maps. Validation was performed through a retrospective analysis of over 100 stroke cases, comparing the recalculated perfusion maps with standard clinical interpretations. Sensitivity, specificity, and accuracy were the primary metrics assessed.Results The recalculation program demonstrated acceptable differences in the identification of the CVV, CVF, MTT, and TTP compared to conventional analysis methods.Abstract A56 Figure 1Conclusion The PWI-DWI Recalculation Program offers a promising advancement in the field of stroke clinical research. By addressing the limitations of current standard forms, this program could benefit researchers and physicians by allowing future research to be conducted at no cost. Additional studies are underway to apply this program to larger patient populations and incorporate additional imaging modalities.Conflict of Interest Yes Medtronics