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Background People living in prison (PLP) are at increased risk of blood-borne viruses (BBVs) and sexually transmitted infections (STIs), while often experiencing limited access to prevention information and services; nevertheless, prisons represent a key setting for reaching vulnerable and underserved populations.For several years, the penitentiary health service at San Vittore Prison has established a structured collaboration with dedicated staff of LILA Milano, a community-based HIV/STI organization. LILA staff access the facility weekly to provide information about prevention, individual counseling, anonymous rapid testing for HIV, HCV, HBV and syphilis, vaccination counseling and promotion of vaccine uptake. This study describes this collaborative model as a best practice to address prevention and care gaps among PLP.Material and Methods At entry into San Vittore, all newly incarcerated individuals are offered voluntary screening for HIV, HCV, HBV, and syphilis by the penitentiary health service. LILA Milano subsequently contacts individuals who declined testing at intake, offering community-led, anonymous rapid testing and counseling. The organization also conducts group educational sessions in different prison wings, provides individual counseling to PLP living with HIV and/or viral hepatitis, and supports linkage to care when needed. In 2025, LILA Milano actively contributed to promoting a prison-wide vaccination campaign (HBV, HPV, DTaP-IPV).Results In 2025, 3.028 individuals entered the San Vittore Prison. Of these, 1.147 (37.9%) accepted voluntary screening at intake ( table 1). Among those not tested at entry, LILA Milano performed 120 rapid HIV tests (6.4%), 107 HCV tests (5.7%), 108 HBV tests (4.6%), and 86 syphilis tests (4.6%). Overall, 123 PLP underwent community-based testing in 2025; 96 (78%) were male and 44 (35.8%) were foreign nationals (table 2). Unlike previous years, no reactive results were detected. Regarding vaccination, active promotion by LILA Milano significantly supported uptake during the 2025 campaign: the number of vaccine doses administered increased from 92 in the first semester to 2.702 in the second semester (table 3).Conclusions A long-standing collaboration between prison health services and a community-based organization can effectively reach PLP who are otherwise harder to engage. Through diversified strategies - group education, individual counseling, anonymous rapid testing, and vaccination promotion - this integrated model increased screening coverage and markedly improved vaccine uptake, contributing to better health outcomes and wellbeing among PLP.Abstract SC2 Table 1–3