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Prophylactic human papillomavirus (HPV) vaccination is a primary preventive measure aimed at preventing HPV-related lesions and cancers by preventing HPV infection. With the continuous update of global data on HPV vaccination, seven academic societies—including the Chinese Society for Colposcopy and Cervical Pathology of China Healthy Birth Science Association and Chinese Society of Gynecological oncology, Chinese Medical Association and others—have jointly developed the Guidelines for the Clinical Application of Prophylactic Human Papillomavirus (HPV) Vaccines in China (2025 Edition). This guideline was based on the previous Chinese Expert Consensus on the Clinical Application of Human Papillomavirus Vaccines1 as well as current evidence-based medical research, China’s national conditions and clinical realities. The recommended categories of this guideline are listed in table 1.