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Objective To develop a comprehensive, step-by-step guide for conducting successful field studies on occupational heat stress and kidney function in hot climates, specifically tailored to low-resource settings, in the context of the developing world.Material and Methods This guide was developed through synthesis of extensive field experience and critical review of a broad range of literature. Key components of successful workplace-based investigations were identified. The process focused on practical challenges and methodological needs, from early planning to reporting results.Results The guide emphasizes initiating research with full stakeholder engagement and contextual site visits. A clear research question and study population must be defined. It outlines, workplace visits providing contextual understanding and interaction with stakeholders before determining priority study groups and for logistical planning for field data collectionLocal resources for health care and laboratory analysis need to be specified and considered. The guide provides recommendations for assessing environmental heat, workload, heat strain, hydration, and a selection of health outcomes as well as questionnaire development. Detailed checklists and basic questionnaires are provided.Finally, an approach to analysis and report writing is emphasized.Conclusion This guide offers a structured approach to occupational heat stress and kidney health research, enhancing comparability across settings and strengthening evidence to inform effective interventions for prevention of workplace heat stress and related health risks.Acknowledgements We acknowledge the La Isla Network´s field team. Without their experiences on the ground and the multidisciplinary expertise, this protocol paper could not have been written.Funding A Belmont Forum grant, PREP provided external funding. In-kind funding was also obtained from the University of Gothenburg and Lund University, Sweden, the University of Birmingham, UK, and from La Isla Network.