Previously unrecognised gene fusions across diverse solid tumours identified by anchored multiplex RNA sequencing

Gene fusions play a pivotal role as both diagnostic and therapeutic biomarkers. They arise from chromosomal rearrangements, such as translocations, deletions, inversions or altered transcription events, resulting in fusion proteins that drive oncogenesis across diverse cancer types.1 Because many clinically relevant fusions are cryptic or involve unknown partners, RNA-based approaches are increasingly essential for their detection. Here, we highlight seven previously unreported gene fusions across diverse solid tumours using RNA-based anchored multiplex polymerase chain reaction (PCR) (AMP) next-generation sequencing (NGS), all confirmed using reverse-transcription PCR with fusion-specific primers (table 1). Collectively, these findings expand the spectrum of gene rearrangements with potential diagnostic and biological relevance.